| 货号 | 9393S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | CRMP-2 Antibody detects endogenous levels of total CRMP-2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5). |
| 存放说明 | -20C |
| 计算分子量 | 55-65 |
Western blot analysis of extracts from mouse and rat brain using CRMP-2 Antibody. Western blot分析大鼠和小鼠大脑提取物，使用的抗体是CRMP-2 Antibody 兔多抗。 | |
Confocal immunofluorescent analysis of PC-12 cells using CRMP-2 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析PC-12细胞，使用CRMP-2 Antibody 兔多抗（绿色）进行标记。肌动蛋白纤丝用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5® 4084(荧光DNA染料)。 |
