| 货号 | 2818S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Cripto Antibody detects endogenous levels of total mouse Cripto protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Cripto, also known as teratocarcinoma derived growth factor 1 (TDGF-1), belongs to the EGF-CFC family of proteins. Members of this family are characterized by an N-terminal signal peptide, a conserved cysteine rich domain (CFC motif), and a short hydrophobic carboxy-terminal tail that contains GPI cleavage and attachment sites. The GPI moiety anchors Cripto and family members to the extracellular plasma membrane (1). An O-linked fucosylation site within the EGF-like motif is required for Cripto and related family members to perform their function as co-receptors for TGF-β-related ligands such as Nodal and Vg1/GDF1 (2,3). Soluble forms of Cripto can be produced - these contain intact EGF and CFC domains, and are thought to have paracrine activities, as opposed to the autocrine activity of Cripto functioning as a coreceptor (4). Understanding of this paracrine activity is not complete, but it is proposed that Cripto may act as co-ligand for Nodal (3). Cripto is an important modulator of embryogenesis and oncogenesis (4). It is highly expressed in early embryos, and in embryonic stem (ES) cells where it is involved in cardiomyocytic differentiation and acts as a negative regulator of neurogenesis (5-7). Transient activation of Cripto is essential for the capacity of stem cell self-renewal and pluripotency in ES cells, and in some adult derived stem cells (8). Signaling through Cripto can also stimulate other activities that promote tumorigenesis such as stimulation of proliferation, cell motility, invasion, angiogenesis and epithelial-mesenchymal transition (EMT) (9-11). Cripto is highly expressed in a broad range of tumors, where it acts as a potent oncogene. |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Western blot analysis of extracts of F9 and mouse embryonic stem (ES) cells using Cripto Antibody. 使用Cripto抗体对F9和小鼠胚胎干细胞（ES）提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of P19 cells using Cripto Antibody (Mouse Specific) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Cripto抗体（鼠特异性）对P19细胞进行激光共聚焦荧光分析（绿色）。使用DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5® #4084 （DNA荧光染料）。 |
