| 货号 | 2832S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | cPLA2 Antibody detects endogenous levels of total cPLA2 protein. This antibody does not cross-react with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity. |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Immunoprecipitation of cPLA2 from untreated and TNF-alpha treated HeLa cells followed by Western blot analysis, using cPLA2 Antibody. 对未处理或TNF-α处理的HeLa细胞使用cPLA2抗体进行免疫沉淀，然后进行Western blot分析。 | |
Western blot analysis of extracts from HeLa cells, untreated or TNF-α-treated (10 ng/ml for 15 minutes), and NIH/3T3 and COS cells, using Phospho-cPLA2 (Ser505) Antibody #2831 (upper) or cPLA2 Antibody (lower). 对HeLa细胞，未处理或10 ng/ml TNF-α处理15分钟，以及NIG/3T3和COS细胞，使用hospho-cPLA2 (Ser505)抗体#2831抗体（上图）或cPLA2抗体（下图）进行Western blot分析。 |
