CP110 Antibody
货号：
12140S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 12140S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The centrosome is an organelle that plays an important role in the mammalian cell cycle. Prior to the onset of mitosis, the single interphase centrosome duplicates only once, creating a pair of daughter centrosomes that will form the two spindle poles after breakdown of the nuclear envelope. Failure to duplicate or overduplication of the centrosome can result in polyploidy and genomic instability (reviewed in 1). Centrosomal protein of 110 kDa (CP110) is a cyclin-dependent kinase (CDK) substrate that plays a critical role in promoting the duplication of centrosomes and correct spindle formation (2). In addition, CP110 has been shown to interact with calmodulin (CaM) and centrin to regulate the progression through cytokinesis (3), and with Cep97 and Cep290 to regulate the formation of primary cilia (4,5). CP110 expression is induced in G1/S with peak expression during S-phase. Degradation of CP110 is mediated by cyclin F in G2-phase and is required for normal progression into M-phase (6).中心体是一个在哺乳动物细胞周期中发挥重要功能的细胞器。在有丝分裂开始之前，单面中心体仅会复制一次，产生一组姐妹染色单体会在细胞核分裂后形成两个纺锤体的两极。中心体没有复制或过度复制都会形成多倍体和基因组不稳定（1）。110kDa中心体蛋白（CP110）是一个细胞周期激酶（CDK）底物能够促进中心体复制和纺锤体的正确形成（2）。除此以外，已经证明CP110可以与钙调蛋白（CaM）和中心体蛋白结合通过胞质分裂调控细胞周期进程（3），CP110也可以和Cep97和Cep290结合调控原纤毛形成（4,5）。CP110在G1/S期开始表达，在S期表达最高。CP110在G2期通过cyclin F降解，CP110降解对于细胞正常进入M期是至关重要的（6）。 |
| 存放说明 | -20C |
| 计算分子量 | 130 |
参考图片Western blot analysis of extracts from various cells lines using CP110 Antibody.使用CP110抗体对多种细胞提取物进行western blot分析。 |
Western blot analysis of extracts from T98G cells, serum starved for 48 hr and then either untreated (-) or treated with 10% serum for 24 hr (+), using CP110 Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).使用CP110抗体(上)或α-Actinin (D6F6) XP® Rabbit mAb #6487 (下)对血清饥饿处理48h然后不处理（-）或10%血清处理24h（+）的T98G细胞提取物。 |
