| 货号 | 3502S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Cortactin Antibody detects endogenous levels of total cortactin protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4). Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8). |
| 存放说明 | -20C |
| 计算分子量 | 80, 85 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and membrane localization, using Cortactin Antibody. 使用Cortactin Antibody，免疫组化分析人类乳腺癌组织石蜡切片，结果显示在细胞质和膜上定位。 | |
Western blot analysis of extracts from C6, COS and HeLa cells, untreated or hydrogen peroxide (H2O2)-treated, using Cortactin Antibody. 使用Cortactin Antibody，免疫印迹（Western Blot）分析C6、COS和HeLa细胞系中Cortactin蛋白水平。 |
