Cool1/βPix Antibody
货号：
4515S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 4515S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αpix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/βPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/βPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4). Phosphorylation of cool1/βPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/βPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/βPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/βPix and activation of cdc42 (6).Cool/Pix蛋白是由定位于粘着斑的鸟嘌呤核苷交换因子（guanine nucleotide exchange factors，GEFs）家族组成。该家族包含有两个亚型：cool2/αpix and cool1/βPix，后者有两个剪切变异体，其区别是在它们的羧基终端(1)。像其它GEFs，Cool1/βPix蛋白有一个DH (Dbl homology)功能域，其能允许小GTPases的结合和GDP/GTP的交换，和一个PH (Pleckstrin homology) 功能域，其在亚细胞的定位中有重要作用。Cool1/βPix蛋白也有一个SH3功能域，它结合cdc42和Rac激酶的下游效应因子PAK激酶(3,4)。通过MAPK信号的下游 PAK2蛋白使cool1/βPix蛋白的磷酸化改变包括 PAK2和cool-1/βPix的复合物的定位，在生长因子作用下调节生长锥（growth cone）的形成(4)。生长因子通过cool1/βPix 蛋白诱导Rac1蛋白的激活之后被显示发现在独立的亚细胞定位(5)。肾小球系膜细胞的Endothelin-1刺激蛋白激酶A(PKA)通路，导致cool-1/βPix蛋白的定位和cdc42蛋白的激活(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 81 |
参考图片Western blot analysis of extracts from Jurkat, NIH/3T3, ZR-75, MCF-7 and COS cells using Cool1/βPix Antibody.使用Cool1/βPix Antibody，免疫印迹（Western Blot）分析Jurkat、NIH/3T3、ZR-75、MCF-7和COS细胞系中Cool1/βPix蛋白水平。 |
