| 货号 | 3512S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-F/IF-IC |
| 目标/特异性 | Connexin 43 Antibody detects endogenous levels of total connexin 43. This antibody does not cross-react with other connexins. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:100) IF-IC (1:75) IHC-F (1:200) |
| 供应商 | CST |
| 背景 | Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 39, 41, 43, 44 |
Western blot analysis of extracts from untreated and PMA-treated C6, COS, HeLa and C2C12 cells using Connexin 43 Antibody.使用Connexin 43抗体对未处理和经过PMA处理的C6, COS，HeLa和C2C12细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse heart using Connexin 43 Antibody in the presence of control peptide (left) or antigen specific peptide (lright).在对照多肽（左）或抗原特异性多肽（右）存在的条件下，使用Connexin 43抗体对石蜡包埋的小鼠心脏组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Connexin 43 Antibody.使用Connexin 43 抗体对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human heart, using Connexin 43 Antibody. (High magnification, inset).使用Connexin 43抗体对石蜡包埋的人心脏组织进行免疫组化分析（高倍镜，嵌入）。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Connexin 43 Antibody.使用Connexin 43抗体对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Confocal immunofluorescent analysis of rat optic nerve and ciliary epithelium using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).See notes for additional testing details.使用Connexin 43 抗体（绿色）和S6核糖体蛋白（54D2）小鼠单抗#2317（红色）对大鼠视神经和纤毛上皮细胞进行激光共聚焦免疫荧光分析。蓝色假色 = DRAQ5® #4084 （DNA荧光染料）。更多的测试细节请查看记录。 | |
Immunohistochemical analysis of frozen mouse heart tissue, using Connexin 43 Antibody.使用Connexin 43 抗体对冷冻的小鼠心脏组织进行免疫组化分析。 | |
Confocal immunofluorescent analysis of confluent COS cells using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Connexin 43 抗体（绿色）和S6核糖体蛋白（54D2）小鼠单抗#2317（红色）对接触抑制的COS细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084 （DNA荧光染料）。 |
