| 货号 | 3256S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | CLK3 Antibody detects endogenous levels of full-length and truncated forms of CLK3 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | The cdc2-like kinase (CLK) family contains at least four highly conserved isoforms: CLK1, CLK2, CLK3 and CLK4 (1,2). CLKs are dual specificity kinases that autophosphorylate on serine, threonine and tyrosine residues and phosphorylate exogenous substrates on serine and threonine residues (2). CLK family members exist as both a full-length catalytically active form and an alternatively-spliced, inactive truncated form (1). A family of highly phosphorylated proteins, called serine and arginine rich (SR) proteins, are phosphorylated by CLKs (3-5). SR proteins are splicing factors that regulate the assembly of the spliceosome, a macromolecular complex where RNA splicing occurs in the nucleus. They are also involved in the selection of splice sites. Thus, CLKs may play important roles in regulating RNA splicing. |
| 存放说明 | -20C |
| 计算分子量 | 59, 17 |
Western blot analysis of extracts from Jurkat, HT29 and PC12 cell lines using CLK3 Antibody. 使用CLK3 Antibody，免疫印迹（Western Blot）分析Jurkat、 HT29和PC12细胞系提取物。 | |
Confocal immunofluorescent analysis of HT-29 cells using CLK3 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用CLK3 Antibody（绿色）标记，共聚焦免疫荧光观察CLK3蛋白在HT-29细胞定位。 Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。 |
