| 货号 | 12417S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | CK1δ Antibody recognizes endogenous levels of total CK1δ protein. This antibody does not cross-react with CK1ε. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α, β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).CK1δ is involved in many cellular processes such as differentiation (5), cell growth and apoptosis (6-8), and control of the circadian rhythm (9). |
| 存放说明 | -20C |
| 计算分子量 | 44 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human CK1δ (CK1δ-Myc; +) or Myc-tagged full-length human CK1ε (CK1ε-Myc; +), using CK1δ Antibody (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). Western blot方法检测293T细胞提取物：空质粒转染（-）或带Myc-标签全长人CK1δ (CK1δ-Myc; +)或带Myc-标签全长人CK1ε (CK1ε-Myc; +)载体转染；使用的抗体是CK1δ Antibody兔多抗（上图）或Myc-Tag (71D10) Rabbit mAb兔单抗 #2278（下图）。 | |
Western blot analysis of extracts from various cell lines using CK1δ Antibody. Western blot 检测多种细胞系提取物，使用的抗体是CK1δ Antibody兔多抗。 |
