| 货号 | 4297S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Checkpoint with forkhead and RING finger domains protein (CHFR) is an E3 ubiquitin-protein ligase that regulates cell cycle progression. In response to microtubule stress, CHFR delays the transition into mitosis by excluding cyclin B1 from the nucleus prior to chromosome condensation (1). Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation (1-4). Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2-deoxycytidine has been reported (4,5).细胞有丝分裂前期检查点蛋白(CHFR)是一种E3泛素蛋白连接酶，它能够调节细胞周期进程。在微管应激反应的作用下，CHFR先于染色体凝聚排除核中的周期蛋白B1从而延迟细胞进入有丝分裂(1)。在一些原发性肿瘤中已检测到CHFR表达的显著减少，而其表达的降低和启动子甲基化有关(1-4)。有研究报道采用微管应激反应试剂诺考达唑和甲基转移酶抑制剂5-叠氮-脱氧胞嘧啶处理能够回复CHFR的表达(4,5)。 |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from various cell lines using CHFR Antibody.Western blot方法检测不同细胞提取物，使用的抗体为CHFR Antibody。 | |
Western blot analysis of extracts from MDA-MB-435 cells, untreated or nocodazole-treated, using CHFR Antibody.Western blot 方法检测未处理和nocodazole处理的MDA-MB-435细胞提取物，使用的抗体为CHFR Antibody。 |
