CENP-T Antibody
货号：
12494S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 12494S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays a critical role in the regulation of transcription and replication of the eukaryotic genome. The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin (2). The greatest divergence between CENP-A and canonical histone H3 occurs in the amino-terminal tail of the protein, which binds linker DNA between nucleosomes and facilitates proper folding of centromeric heterochromatin (3). The amino-terminal tail of CENP-A is also required for recruitment of other centromeric proteins (CENP-C, hSMC1, hZW10), proper kinetochore assembly, and chromosome segregation during mitosis (4).
CENP-A is regarded as the epigenetic mark of the centromere that persists through cell generations (5). Although its presence is necessary, it is not sufficient for formation of functional kinetochores (6). CENP-T, in complex with CENP-W, has recently been shown to form a histone fold, a structure that is capable of association with DNA, and target DNA to the kinetochore (7). Kinetochore attachment is mediated by a long flexible N-terminal region that has been shown to interact with outer proteins of the kinetochore complex (reviewed in 8). Moreover, the CENP-T-W complex has also been shown to interact with the CENP-S-X dimer, to form a heterotetrameric complex that has structural and potentially functional similarity to canonical histones (8). Since CENP-S-X are conserved kinetochore localized proteins, this new complex has been suggested to be a novel centromeric histone.染色质结构调整在真核基因组的转录和复制调控过程中发挥了重要作用。核小体，由四个核心组蛋白组成（H2A, H2B, H3, 和H4），是染色质的主题结构。此外，除了越来越多的翻译后组蛋白修饰以调控染色质结构外，细胞也能通过将经典组蛋白更换成突变型的组蛋白以直接或间接地调整染色质结构（1）。CENP-A，也被称为染色质-相关蛋白CSE4（capping-enzyme suppressor 4-p），是一个关键的组蛋白H3变异体可以替换着丝粒异染色质中的经典组蛋白H3（2）。CENP-A和经典组蛋白H3的最大区别位于蛋白的氨基末端，该区域通过核小体和linker DNA连接，促进着丝粒异染色质的正确折叠(3). CENP-A的氨基末端对于有丝分裂时期，其他着丝粒蛋白(CENP-C, hSMC1, hZW10)的富集，正确地组装着丝粒和染色体分离，也是必须的（4）。CENP-A被认为是着丝粒的表观遗传学标记（5）。尽管它的存在时必须的，但是并不足以形成有功能的着丝粒（6）。CENP-T，和CENP-W形成复合物，最近被发现可以形成一个组蛋白折叠，这是一种足以和DNA结合，促使DNA到达着丝粒的结构（7）。已经发现一个长的灵活的N末端区域可以和着丝粒复合物的外围蛋白结合以介导形成着丝粒附件（8）。此外，CENP-T-W复合物可以和CENP-S-X结合，形成一个异源三聚体，该异源三聚体的结果和可能的功能都与经典组蛋白相似（8）。因为CENP-S-X是保守的着丝粒蛋白，这个新的复合物被认为是一个新发现的着丝粒组蛋白。 |
| 存放说明 | -20C |
| 计算分子量 | 65 |
参考图片Western blot analysis of extracts from various cell lines using CENP-T Antibody.使用CENP-T抗体对多种细胞提取物进行western blot分析。 |
