| 货号 | 11973S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinase 12 (CDK12/CRKRS/CRK7) is composed of a central CTD kinase domain, several proline-rich regions, and several amino-terminal arginine/serine (RS) motifs common to splicing factors (1). CDK12 is ubiquitously expressed and forms a complex with cyclin K that regulates phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (1-4). CDK12 is important for expression of a subset of long genes with high numbers of exons including some regulators of the DNA damage response, such as breast and ovarian cancer type 1 susceptibility protein 1 (BRCA1) and ataxia telangiectasia and Rad3-related (ATR) (3). Depletion of CDK12 results in spontaneous DNA damage and increased sensitivity to DNA damage agents (3). Research studies have shown that CDK12 is recurrently mutated in high-grade ovarian cancer (5,6). In addition, high levels of CDK12 are required to maintain pluripotency of embryonic stem cells (7).细胞周期依赖性激酶12（CDK12/CRKRS/CRK7）由一个位于中心的CTD激酶结构域，若干个富含脯氨酸的区域和若干个amino-terminal arginine/serine (RS)基序共同组成（1）。CDK12广泛表达并和cyclin K组成复合物调控RNA聚合酶II的C-末端区域(CTD)的磷酸化(1-4)。CDK12对于一系列有大量外显子的长基因的表达非常重要，包括一些DNA损伤应激调控因子，例如乳腺癌和卵巢癌1型敏感蛋白(BRCA1)和共济失调毛细血管扩张症以及Rad3-related (ATR) (3).敲除CDK12会引发DNA损伤增加对DNA致损剂的敏感性(3).研究发现在高度的卵巢癌病人体内CDK12周而复始的发生突变(5,6). 并且，高表达的CDK12被用于维持胚胎干细胞的多潜能性(7). |
| 存放说明 | -20C |
| 计算分子量 | 205 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CDK12 isoform 2 (hCDK12-Myc/DDK; +), using CDK12 Antibody.使用CDK12抗体对模拟转染(-)或转染了表达Myc/DDK-tagged full-length human CDK12 isoform 2 (hCDK12-Myc/DDK; +)质粒的293T细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using CDK12 Antibody.使用CDK12抗体对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of CDK12 from PC-3 cell extracts using Normal Rabbit IgG #2729 (lane 2) or CDK12 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CDK12 Antibody.使用Rabbit IgG #2729 (lane 2)或CDK12抗体(lane 3)对PC-3细胞提取的CDK12进行免疫沉淀分析。CDK12抗体用于western blot实验。 |
