| 货号 | 13824S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Cell Death Involved p53-Target (CDIP) is a pro-apoptotic protein induced by genotoxic stress in a p53-dependent manner (1). Indeed, CDIP is a direct target of p53. Inhibition of CDIP impairs p53-mediated apoptosis. Induction of apoptosis by CDIP requires caspase-8 suggesting involvement in the extrinsic apoptotic pathway. CDIP also promotes increased expression of TNF-α capable of triggering apoptosis. Furthermore, expression of CDIP correlates with increased sensitivity of TNF-α-induced apoptosis (2). These studies suggest that CDIP may serve as a marker for apoptotic sensitivity. |
| 存放说明 | -20C |
| 计算分子量 | 24,26 |
Western blot analysis of extracts from U-2 OS cells, untreated (-) or treated with Doxorubicin #5927 (500 nM, overnight; +), using CDIP Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged-transcript of the active form of CDIP protein (hCDIP-Myc/DDK; +), using CDIP Antibody (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Immunoprecipitation of CDIP from RD cell extracts using Normal Rabbit IgG #2729 (lane 2) or CDIP Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CDIP Antibody. | |
Western blot analysis of extracts from RD and A172 cells using CDIP Antibody. |
