| 货号 | 4115S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | CDCP1 Antibody detects endogenous levels of total CDCP1 protein. The 140kDa band is full length, glycosylated CDCP1, the 70kDa band is a cleavage product of CDCP1. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | CDCP1 (CUB domain containing protein, SIMA135) is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. Three CUB domains in its extracellular region are possibly involved in cell adhesion or extracellular matrix interaction (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2- domain containing proteins (3). CDCP1 is a putative marker for hematopoietic stem cells (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 140, 70 |
Western blot analysis of extracts from PC3 and HT29 cells using CDCP1 Antibody.使用CDCP1抗体对PC3和HT29细胞提取物进行Western blot实验分析。 | |
Confocal immunofluorescent analysis of HT-29 cells using CDCP1 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用CDCP1 抗体（绿色）对HT-293 细胞进行激光共聚焦荧光分析实验。蓝色假色=DRAQ5® #4084 (荧光DNA染料). | |
Confocal immunofluorescent analysis of DLD-1 (left) and MCF7 (right) cells using CDCP1 Antibody (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087 (fluorescent DNA dye). |
