Cdc45 Antibody
货号：
3673S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 3673S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to bind to chromatin throughout the cell cycle (1,2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase and formation of the pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases cdk2 and cdc7. Cdc7 phosphorylates MCM proteins bound to chromatin and, in conjunction with CDT1, recruits the replication factor cdc45 (3-5). Cdc45 is required for formation of pre-initiation complexes at the G1/S transition and for activation of replication origins. The level of cdc45 protein expression is associated with the proliferative status of the cell or tissue. Terminally differentiated and senescent cells lack cdc45 and highly proliferative cell lines express high levels of cdc45 (6).哺乳动物细胞的DNA复制启动是一个高度协调的过程，从而保证了每次细胞分裂中基因组只复制一次。复制的起源分散在整个基因组中，其活性主要通过前复制因子和复制因子的连续结合来调控。起始识别复合体(ORC)被认为可以结合整个细胞周期的染色质(1,2)。前复制复合体(Pre-RC)在有丝分裂末期/G1早期随着CDT1和cdc6的结合而形成，允许异源六聚化的MCM2-7复合体的结合。MCM复合体被认为是一种复制解旋酶，而pre-RC的形成参与染色质许可。随后的DNA复制起始需要S期促进激酶cdk2 和cdc7的激活。Cdc7磷酸化结合于染色质的MCM蛋白并连同CDT1招募复制因子cdc45 (3-5)。G1/S转换期前起始复合体的形成和复制起始物的激活需要cdc45的参与。Cdc45蛋白的表达水平和细胞或者组织的增殖状态有关。最终分化的和衰老的细胞缺少cdc45而高增殖能力的细胞系高表达cdc45(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 65 |
参考图片Western blot analysis of extracts from various cell types using Cdc45 Antibody.western blot方法检测不同细胞提取物，使用的抗体为Cdc45 Antibody。 |
