Cdc42 Antibody
货号：
2462S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 2462S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the proteins intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).Rac和Cdc42蛋白是Rho-GTPase家族成员。在哺乳动物类，Rac蛋白存在有三个亚型Rac1、Rac2和Rac3，它们在序列上高度类似。Rac1和Cdc42蛋白是在这个家族中最广泛研究的，它们无处不在的表达。Rac2蛋白在造血起源的细胞中被表达，在大脑中高表达的Rac3蛋白也被发现在许多其它组织。Rac和Cdc42蛋白在细胞骨架的重组、膜运输、转录调控、细胞生长和发育中起到关键的信号角色(1)。GTP的结合刺激Rac/Cdc42蛋白的活性，然而通过蛋白质内部GTPase活性促使GTP水解成GDP，致使它的失活。GTPase激活蛋白(GAPs)辅助GTP的水解，而鸟嘌呤核苷交换因子（guanine nucleotide exchange factors ，GEFs）有助于GDP的转换成GTP 。通过鸟苷酸解离抑制因子(RhoGDI)的结合使得达到另一个调节的水平，这保留了Rho家族GTPases包括Rac和Cdc42，在它们失活的GDP结合水平(2,3)。 |
| 存放说明 | -20C |
| 计算分子量 | 21 |
参考图片Western blot analysis of HEK293, HT29, A431, PL45, THP1, Jurkat, NIH/3T3, L929, C6, H-4-II-B and BAEC cells, using Cdc42 Antibody.使用Cdc42 Antibody，免疫印迹（Western Blot）分析HEK293、HT29、A431、PL45、THP1、Jurkat、NIH/3T3、L929、C6、H-4-II-B和BAEC细胞系Cdc42蛋白水平。 |
