cdc25A Antibody
货号：
3652S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 3652S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The cdc25 protein phosphatase family plays a critical role in activating cyclin-dependent kinases (CDKs) via dephosphorylation of conserved Thr14/Tyr15 inhibitory phosphorylation sites. While cdc25C is primarily responsible for activating CDK1 to overcome the G2/M checkpoint and allow mitotic entry, the primary substrate of cdc25A is CDK2, which, when active, allows progression through the G1/S and intra-S checkpoints (1). Abundance, subcellular localization and activity of cdc25A is tightly controlled by a variety of mechanisms, including phosphorylation, ubiquitination, and inhibitory binding to 14-3-3 proteins. During normal cell cycle progression, elevated c-Myc and E2F transcription factor levels lead to increased cdc25A expression (2). When conditions are favorable for DNA synthesis, cdc25A and CDK2 form an activation loop, wherein each activates the other enzyme (1). DNA damage, on the other hand, leads to multisite phosphorylation at inhibitory sites (Ser123, Ser177, Ser278, Ser292, and Thr506) by Chk1 and Chk2, which result in 14-3-3 binding and ubiquitin-mediated degradation (3,4).cdc25蛋白磷酸酶家族在激活周期蛋白依赖性激酶(CDKs)的过程中发挥关键作用，这种作用主要通过去磷酸化保守的苏氨酸14/15位点。cdc25C是激活CDK1从而克服G2/M期检验点促进有丝分裂的首要原因。cdc25A的主要底物是CDK2，激活状态的CDK2允许G1/S期和S期检验点的进展(1)。各种机制如磷酸化、泛素化和14-3-3蛋白结合抑制等紧密控制cdc25A的丰度、亚细胞定位和活性。在正常的细胞周期进程中，转录因子c-Myc和E2F水平的升高可以导致cdc25A表达的增加(2)。当条件有利于DNA合成时，cdc25A和CDK2组成一个激活回路，其中分别激活其它的酶类(1)。另一方面，DNA损伤可以通过Chk1和Chk2引起多个抑制性位点(Ser123, Ser177, Ser278, Ser292, and Thr506)的磷酸化，最终导致14-3-3蛋白结合和泛素介导的降解(3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 70 |
参考图片Western blot analysis of extracts from asynchronous (lane 1) or synchronized (lanes 2-4) NIH/3T3 cells, using Phospho-cdc25A (Thr506) Antibody #3654 (upper) or cdc25A Antibody (lower). Cells were synchronized by mitotic shake-off and replated for the indicated times. western blot 方法检测非同步（泳道1）和同步的（泳道2-4）NIH/3T3细胞，使用的抗体为Phospho-cdc25A (Thr506) Antibody #3654 (上图) 和cdc25A Antibody (下图)。细胞同步化在脱离有丝分裂后发生，并回复到固定的时相。 |
