CDC20 Antibody
货号：
4823S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 4823S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The cell division cycle demands accuracy to avoid the accumulation of genetic damage. This process is controlled by molecular circuits called "checkpoints" that are common to all eukaryotic cells (1). Checkpoints monitor DNA integrity and cell growth prior to replication and division at the G1/S and G2/M transitions, respectively. The cdc2-cyclin B kinase is pivotal in regulating the G2/M transition (2,3). Cdc2 is phosphorylated at Thr14 and Tyr15 during G2-phase by the kinases Wee1 and Myt1, rendering it inactive. The tumor suppressor protein retinoblastoma (Rb) controls progression through the late G1 restriction point (R) and is a major regulator of the G1/S transition (4). During early and mid G1-phase, Rb binds to and represses the transcription factor E2F (5). The phosphorylation of Rb late in G1-phase by CDKs induces Rb to dissociate from E2F, permitting the transcription of S-phase-promoting genes. In vitro, Rb can be phosphorylated at multiple sites by cdc2, cdk2, and cdk4/6 (6-8). DNA damage triggers both the G2/M and the G1/S checkpoints. DNA damage activates the DNA-PK/ATM/ATR kinases, which phosphorylate Chk at Ser345 (9), Chk2 at Thr68 (10) and p53 (11). The Chk kinases inactivate cdc25 via phosphorylation at Ser216, blocking the activation of cdc2.细胞分裂周期的维持需要准确避免基因损伤的累积。这一过程由真核细胞中普遍存在的所谓检验点分子环路控制(1)。在G1/S和G2/M转换期，检验点先于复制和分化分别监控DNA完整性和细胞生长。cdc2-cyclin B激酶是调节G2/M过渡的重要激酶(2,3)。Cdc2的苏氨酸14位和酪氨酸15位在G2期可被Wee1和Myt1两个激酶磷酸化，从而失活。肿瘤抑制蛋白视网膜母细胞瘤蛋白(Rb)通过G1末期检验点控制细胞进程，是G1/S 转换的重要调节子(4)。在G1期的早中期， Rb蛋白与转录因子E2F结合并抑制其表达(5)。G1期晚期，CDKs磷酸化Rb诱导其与E2F解离，S期促进基因得以转录。在体外，Rb可以被cdc2, cdk2, and cdk4/6进行多位点磷酸化 (6-8)。DNA损伤可以触发G2/M及G1/S检验点。DNA损伤能够激活DNA-PK/ATM/ATR激酶，后者能够使Chk丝氨酸345位 (9)，Chk2苏氨酸68位和p53发生磷酸化(11)。Chk激酶通过丝氨酸216位失活cdc25，从而阻断cdc2的激活。 |
| 存放说明 | -20C |
| 计算分子量 | 51 |
参考图片Western blot analysis of extracts from various cell lines using CDC20 Antibody.western blot 方法检测不同细胞系提取物，使用的抗体为CDC20 Antibody。 |
