| 货号 | 3383S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Cathepsin B (H190) Antibody (Mouse Preferred) detects endogenous levels of total cathepsin B protein. Cathepsin B (G60) Antibody #3373 produces a stronger signal for human cathepsin B on western blot, while Cathepsin B (H190) Antibody is preferred for the mouse protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Cathepsin B (CSTB), part of the papain family of proteases, is a widely expressed lysosomal cysteine endopeptidase (1,2). Like other family members, cathepsin B is produced from a larger precursor form, pro-cathepsin B, which in its glycosylated form runs at approximately 40 kDa on SDS-PAGE, and is then activated by cleavage of a 62-amino acid pro-peptide. High levels of cathepsin B are found in macrophages and osteoclasts, as well as various types of cancer cells, including lung, colon, prostate, breast, and stomach. In addition, expression of cathepsin B has been associated with multiple sclerosis (3), rheumatoid arthritis (4), and pancreatitis (5). While generally localized to lysosomes, in cancer alterations can lead to its secretion (6). Its role in tumor progression is thought to involve promotion of basement membrane degradation, invasion and metastasis (7,8). Expression can correlate with poor prognosis for a variety of forms of cancer (9-13). |
| 存放说明 | -20C |
| 计算分子量 | 39-42 |
Western blot analysis of HeLa cells, mock transfected or transfected with mouse cathepsin B, using Cathepsin B (H190) Antibody. Western blot分析HeLa细胞，空载体转染或转染小鼠cathepsin B，使用的抗体是Cathepsin B (H190) Antibody 兔多抗。 | |
Western blot analysis of extracts from various cell lines using Cathepsin B (H190) Antibody. Western blot分析多个细胞系，使用的抗体是Cathepsin B (H190) Antibody 兔多抗。 |
