| 货号 | 3373S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Cathepsin B (G60) Antibody detects endogenous levels of total human cathepsin B protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Cathepsin B (CSTB), part of the papain family of proteases, is a widely expressed lysosomal cysteine endopeptidase (1,2). Like other family members, cathepsin B is produced from a larger precursor form, pro-cathepsin B, which in its glycosylated form runs at approximately 40 kDa on SDS-PAGE, and is then activated by cleavage of a 62-amino acid pro-peptide. High levels of cathepsin B are found in macrophages and osteoclasts, as well as various types of cancer cells, including lung, colon, prostate, breast, and stomach. In addition, expression of cathepsin B has been associated with multiple sclerosis (3), rheumatoid arthritis (4), and pancreatitis (5). While generally localized to lysosomes, in cancer alterations can lead to its secretion (6). Its role in tumor progression is thought to involve promotion of basement membrane degradation, invasion and metastasis (7,8). Expression can correlate with poor prognosis for a variety of forms of cancer (9-13). |
| 存放说明 | -20C |
| 计算分子量 | 39-42 |
Western blot analysis of extracts from various cell lines using Cathepsin B (G60) Antibody. Western blot分析多个细胞系提取物，使用的抗体是Cathepsin B (G60) Antibody 兔多抗。 | |
Western blot analysis of extracts from secreted protein from HL-60 cells, untreated or treated overnight with TPA #9905 (20 nM), using Cathepsin B (G60) Antibody. Western blot分析HL-60细胞分泌蛋白提取物（未处理，或20 nM TPA #9905处理过夜），使用的抗体是Cathepsin B (G60) Antibody 兔多抗。 |
