Caspase-9 Antibody (Mouse Specific)
货号：
9504S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 9504S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Caspase-9 Antibody (Mouse Specific) detects endogenous levels of both full length mouse caspase-9 (49 kDa) and the large fragment of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). The antibody does not cross-react other caspases. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 37, 39, 49 |
参考图片Western blot analysis of extracts from NIH/3T3 cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Caspase-9 Antibody. p39: caspase-9 cleaved at Asp368. p37: caspase-9 cleaved at Asp353. Western blot分析未处理、staurosporine(星胞菌素)(1 µM) 或 cytochrome(细胞色素C)(0.25 mg/ml)处理的NIH/3T3 细胞，抗体采用Caspase-9 Antibody p39: caspase-9 Asp368处裂解获得. p37: caspase-9 Asp353处裂解获得。 |
