| 货号 | 12283S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | CARD9 Antibody (Mouse Preferred) recognizes endogenous levels of total CARD9 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | CARD9 is a caspase recruitment domain (CARD)-containing adaptor protein expressed by myeloid cells (1,2). It is required for antifungal immunity downstream of pathogen detection by C-type lectin receptors (CLRs) such as Dectin-1 (3,4). Recognition of carbohydrates on fungal cell walls by CLRs leads to activation of the tyrosine kinase Syk, followed by activation of PKCδ (5,6). PKCδ phosphorylates CARD9, enabling the assembly of a complex containing CARD9 and Bcl10 (6). This complex activates NF-κB, resulting in upregulation of inflammatory cytokines important for initiation of adaptive immunity (3,4,6,7). CARD9 was also shown to be important for the induction of IL-1β, downstream of the viral nucleic acid sensor RIG-I, as well as for the generation of reactive oxygen species important for bacterial killing by macrophages (2,8). |
| 存放说明 | -20C |
| 计算分子量 | 64 |
Western blot analysis of extracts from Raw 264.7 and JAWSII cells using CARD9 Antibody (Mouse Preferred).Western blot分析RAW264.7和JAWSII细胞的细胞提取物，使用抗体是CARD9 Antibody (Mouse Preferred). | |
Immunoprecipitation of CARD9 from Raw 264.7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or CARD9 Antibody (Mouse Preferred) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CARD9 Antibody (Mouse Preferred).对RAW264.7细胞的细胞提取物中的CARD9进行免疫共沉淀，使用抗体是Normal Rabbit IgG #2729 (泳道2)或 CARD9 Antibody (Mouse Preferred) (泳道 3)。泳道1为10%的加入量。Western blot 分析使用抗体是 CARD9 Antibody (Mouse Preferred)。 |
