| 货号 | 4146S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Calpastatin Antibody detects endogenous levels of total calpastatin protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Calpain is a thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 in a calcium dependent manner (2). In particular, calpastatin binds and inhibits calpain when calcium levels are high and is released when calcium levels go down. Calpastatin contains five domains, a unique N-terminal domain L with no inhibitory effects and four homologous domains (CAST 1-4) that can inhibit heterodimeric calpain 1 and 2 (3). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from A20 and MDA-MB-231 cells using Calpastatin Antibody. 对A20和MDA-MBPC12细胞裂解液使用Calpastatin Antibody进行Western blot分析。 |
