Calpain 1 Large Subunit (Mu-type) Antibody
货号：
2556S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 2556S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Calpain 1 Large Subunit (Mu-type) Antibody detects endogenous levels of total calpain 1 (large subunit) protein. The antibody detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1). |
| 存放说明 | -20C |
| 计算分子量 | 75, 80 |
参考图片Western blot analysis of extracts from Jurkat and U937 cells, using Calpain 1 Large Subunit (Mu-type) Antibody.使用Calpain 1 Large Subunit (Mu-type)抗体对Jurkat和U937细胞提取物进行western blot分析。 |
