| 货号 | 2295S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | C/EBPα Antibody detects endogenous levels of total C/EBPα protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4). |
| 存放说明 | -20C |
| 计算分子量 | 42, 28 |
Western blot analysis of extracts from THP1 and NIH/3T3-L1 cells using C/EBPα Antibody. 对THP1和NIH/3T3-L1细胞抽提液使用C/EBPalpha Antibody进行Western blot分析。 | |
Confocal immunofluorescent image of 3T3-L1 cells labeled with C/EBPalpha Antibody (green) showing nuclear localization in differentiated cells. 对3T3-L1细胞使用C/EBPalpha Antibody（绿色）进行共聚焦免疫荧光分析，显示分化细胞的胞核定位。 |
