| 货号 | 3769S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | BNIP3 Antibody (Rodent Specific) detects endogenous levels of total BNIP3 protein from mouse and rat. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | BNIP3 (Bcl-2/E1B-19kDa interacting protein 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain and a carboxyl terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kDa, it runs anomalously on SDS-PAGE and includes a band of around 60 kDa that may be a dimeric form that is not reduced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-19kDa. BNIP3 is distinct from other Bcl-2 family members that contain only the BH3 domain in that the TM domain, and not the BH3 domain, is required for mitochondrial targeting and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6,7), although the relationship between BNIP3 and these processes is not fully understood. Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1α (8-10). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (11-14). |
| 存放说明 | -20C |
| 计算分子量 | 22-28, 50-55 |
Western blot analysis of HeLa cells, mock transfected or transfected with mouse BNIP3, CTLL-2, RAW 264.7 and BaF3 cells using BNIP3 Antibody (Rodent Specific). Western blot 方法检测HeLa细胞提取物：空质粒转染（-）或小鼠 BNIP3、CTLL-2、RAW 264.7和BaF3细胞转染；使用抗体为BNIP3 Antibody (Rodent Specific)。 | |
Western blot analysis of extracts from C2C12 cells, untreated (-) or cobalt chloride-treated (100 μM; overnight) using BNIP3 Antibody (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
