| 货号 | 2762S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Bcl-xL Antibody detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
Western blot analysis of extracts from Jurkat (human), NIH/3T3 (mouse) or C6 (rat) cells, untreated or etoposide-treated (25 µM, Jurkat) or staurosporine-treated (1 µM, NIH/3T3 and C6), using Bcl-xL Antibody. 用Bcl-xL Antibody经Western blot 分析未处理和经处理的Jurkat (人源)、NIH/3T3 (小鼠源)或C6 (大鼠)细胞的细胞提取液。 etoposide处理 (25 µM, Jurkat) 或者 staurosporine处理(1 µM, NIH/3T3 和C6)的。 | |
Immunoprecipitation of Bcl-xL from Jurkat cell extracts, using Bcl-xL Antibody. Lane 1 is the lysate control; lane 2 is antibody alone and lane 3 is antibody plus lysate. 免疫共沉淀Jurkat细胞提取液中的 Bcl-xL 蛋白，所用抗体为Bcl-xL Antibody。泳道 1 是提取液，作为对照; 泳道 2是只有抗体 ，泳道3 是抗体加裂解液。 |
