| 货号 | 12822S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | BCAT1 Antibody recognizes endogenous levels of total BCAT1 protein. This antibody cross-reacts with BCAT2. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | BCAT1 and BCAT2 are cytosolic and mitochondrial branched chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids, reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from U-2 OS, MOLT-4 and Jurkat cells using BCAT1 Antibody. Western blot方法检测U-2 OS，MOLT-4和Jurkat 细胞提取物，使用抗体为BCAT1 Antibody。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either Myc/DDK-tagged full-length human BCAT1 (hBCAT1-Myc/DDK; +) or Myc/DDK-tagged full-length human BCAT2 (hBCAT2-Myc/DDK; +), using BCAT1 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Western blot方法检测293细胞提取物：空质粒转染（-）或带Myc/DDK-标签全长人BCAT1 (hBCAT1-Myc/DDK; +)或Myc/DDK-标签全长人BCAT2 (hBCAT2-Myc/DDK; +)载体转染；使用抗体为BCAT1 Antibody（上图），DYKDDDDK Tag (9A3) Mouse mAb 鼠单抗8146（中图），或GAPDH (D16H11) XP® Rabbit mAb兔单抗 #5174（下图）。 |
