| 货号 | 9292S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Bad Antibody detects endogenous levels of total Bad protein. The antibody does not cross-react with related proteins. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A) or Bad (S112A/S136A), untreated, TPA-treated or forskolin-treated, using Phospho-Bad (Ser112) Antibody #9291 (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Bad Antibody (bottom). Western blot（免疫印迹）分析转染了野生型Bad、Bad (Ser112A)、Bad (S136A)、Bad (S112A/S136A)的293 细胞的细胞提取液，未经处理或经TPA或者forskolin处理的，用Phospho-Bad (Ser112) Antibody #9291 (上图)，Phospho-Bad (Ser136) Antibody #9295(中间图) 或者Bad Antibody #9292 (下图)研究。 | |
Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Bad Antibody (right) or Phospho-Bad (Ser112) Antibody #9291 (left). | |
Western blot （免疫印记），用Bad Antibody (右图) 或者 Phospho-Bad (Ser112) Antibody #9291 (左图)研究未经过处理或者经过TPA处理的COS 细胞的细胞提取液。 |
