| 货号 | 2737S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5).在基因组应激中，ATR相互作用蛋白(ATRIP)结合并被DNA损伤和检验点激活激酶ATR(共济失调毛细血管扩张症兼Rad3相关激酶)磷酸化。ATR和ATRIP在检验点信号通路中是一体的并在DNA修复反应中起重要作用(1-3)。RPA包被的单链DNA处ATRIP和RPA (复制蛋白A)的直接相互作用引起磷酸化ATR/ATRIP招募从而导致复制叉停滞和DNA损伤(3)。ATR/ATRIP协同重要的调节蛋白如Rad17 和9-1-1 复合物调整DNA修复和细胞周期进程(4)。TOPBP1(拓扑异构酶II结合蛋白)可以激活ATR与ATRIP的相互作用，这表明ATRIP可能调节ATR的定位和活性(5)。 |
| 存放说明 | -20C |
| 计算分子量 | 82 (isoform 1) |
Western blot analysis of extracts from Jurkat and HEL cells using ATRIP Antibody. | |
Confocal immunofluorescent analysis of HeLa cells using ATRIP Antibody (green). Actin filaments have been labeled with Alexa Fluor®555 phalloidin (red). |
