| 货号 | 2630S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Atg5 Antibody detects endogenous levels of total Atg5. The observed band represents the Atg12-Atg5 conjugated form, but the antibody likely reacts with free Atg5 as well. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from PANC1, HeLa and HT1080 cell lines using Atg5 Antibody. Western blot 分析PANC1、HeLa和HT1080细胞的提取物，使用的抗体是Atg5 Antibody。 |
