| 货号 | 5262S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Atg4C Antibody detects endogenous levels of total Atg4C protein. A band of unknown origin is detected at 23kDa. The intensity of this band is reduced by treatment with Atg4C siRNA. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes (2). Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologues (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4) (3-5). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue (6). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Western blot analysis of extracts from various cell lines using Atg4C Antibody. Western blot分析各种细胞系的细胞提取物，使用抗体是Atg4C Antibody。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg4C siRNA I (+), using Atg4C Antibody #5262 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg4C Antibody confirms silencing of Atg4C expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (未结合) #6568 (-)或 SignalSilence® Atg4C siRNA I (+)，使用抗体是Atg4C Antibody #5262 (上图)或 β-Tubulin (9F3) Rabbit mAb 兔单抗#2128(下图)。 Atg4C Antibody 验证Atg4C表达的沉默，β-Tubulin (9F3) Rabbit mAb 兔单抗用来检测内参。 |
