| 货号 | 5504S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Atg14 Antibody detects endogenous levels of total Atg14 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes.These proteins are involved in the formation of autophagosomes, which are cytoplasmic vacuoles that are delivered to lysosomes for degradation. The class III type phosphoinositide 3-kinase (PI3K) Vps34 regulates vacuolar trafficking and autophagy (4,5). Multiple proteins associate with Vsp34, including p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon (6-12). Atg14L and Rubicon were identified based on their ability to bind to Beclin-1 and participate in unique complexes with opposing functions (9-12). Rubicon, which localizes to the endosome and lysosome, inhibits Vps34 lipid kinase activity; knockdown of Rubicon enhances autophagy and endocytic trafficking (11,12). In contrast, Atg14L localizes to autophagosomes, isolation membranes, and ER and can enhance Vps34 activity. Knockdown of Atg14L inhibits starvation-induced autophagy (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Atg14 siRNA I #6286 (+) or SignalSilence® Atg14 siRNA II #6287 (+), using Atg14 Antibody #5504 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg14 Antibody confirms silencing of Atg14 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® Atg14 siRNA I #6286 (+)或SignalSilence® Atg14 siRNA II #6287 (+)转染，使用的抗体是Atg14 Antibody #5504（上图）或 β-Tubulin (9F3) Rabbit mAb 兔单抗#2128 （下图）。以 β-Tubulin (9F3) Rabbit mAb 兔单抗作为加样对照，Atg14 Antibody 证实Atg14 表达的沉默。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a GFP-Atg14 construct (+), using Atg14 Antibody. GFP-Atg14 construct was kindly provided by Dr. Qing Zhong, University of California, Berkeley CA. Western blot 分析293T细胞的提取物，对照转染(-)或GFP-Atg14 construct (+)转染，使用的抗体是Atg14 Antibody。GFP-Atg14质粒由 Dr. Qing Zhong, University of California, Berkeley CA友情提供。 |
