| 货号 | 2011S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Atg12 Antibody (Mouse Specific) detects endogenous levels of total free and Atg5 bound Atg12 proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 16, 55 |
Western blot analysis of extracts from A20, NIH/3T3, and C2C12 cell lines using Atg12 Antibody (Mouse Specific). Western blot 分析A20、NIH/3T3和C2C12细胞的细胞提取物，使用抗体是Atg12 Antibody（鼠特异性的）。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or chloroquine-treated (right), using Atg12 Antibody (Mouse Specific) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光试验分析NIH/3T3细胞，未处理（左图）或氯喹处理（右图），使用抗体是Atg12 Antibody (Mouse Specific) (绿色)。肌动蛋白微丝使用Alexa Fluor® 555 phalloidin标记（红色）。蓝色伪彩=DRAQ5® #4084（荧光DNA染料）。 |
