| 货号 | 2010S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Atg12 Antibody (Human Specific) detects endogenous levels of total free and Atg5 bound Atg12 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 16, 55 |
Western blot analysis of extracts from various cell lines using Atg12 Antibody (Human Specific). Western blot 分析多种细胞系的细胞提取物，使用抗体是Atg12 Antibody（人特异性的）。 | |
Confocal immunofluorescent analysis of HCT-116 cells, untreated (upper) or chloroquine-treated (lower), using Atg12 Antibody (Human Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光试验分析HCT-116细胞，未处理（上图）或氯喹处理（下图），使用抗体是Atg12 Antibody（人特异性的）（绿色）。蓝色伪彩=DRAQ5® #4084（荧光DNA染料）。 |
