| 货号 | 8581S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | APR3 Antibody recognizes endogenous levels of total APR3 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Apoptosis related protein 3 (APR3), also known as C2orf28, is a membrane protein identified in HL-60 cells treated with all-trans retinoic acid (ATRA) and was later found to be induced by ATRA in other sensitive cell lines (1,2). APR3 is also up-regulated by NFAT and NF-κB activities (3). Regulation of APR3 by ATRA suggests a role in cell differentiation, but the mechanism of action is still unclear. Overexpression of APR3 can inhibit proliferation by inducing G1/S cell cycle arrest and decreasing expression of cyclin D1 (2). APR3 has also been shown to interact with NELL-1 to regulate osteoblast differentiation (4). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Western blot analysis of extracts from HL-60 cells, untreated or treated with retinoic acid for the indicated times (RA; 1 μM), using APR3 Antibody. Western blot 免疫印记。用APR3 Antibody分析未经处理和经过视黄酸处理一定时间 (RA; 1 μM)的HL-60细胞的细胞提取液。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with an expression construct encoding tagged APR3 (+), using APR3 Antibody. The construct was kindly provided by Drs. Timothy Peterson and Erin OShea, Harvard University, Boston MA. Western blot 免疫印记分析转染了对照(-) 或具有APR3 (+)表达标签的质粒的293T细胞的细胞提取液，所用抗体为APR3 Antibody。质粒是由Drs. Timothy Peterson 和Erin OShea，Harvard University，Boston MA赠与。 |
