| 货号 | 2452S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | APP Antibody detects endogenous levels of several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP695, APP770 and APP751. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Amyloid β (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-β), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-β fragments form the main components of amyloid plaques in Alzheimer disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent and peaks during G2/M-phase (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 100 to 140 |
Western blot analysis of extracts from HeLa (human), 3T3L1 (mouse) or C6 (rat) cells, untreated or nocodazole-treated (1 µg/ml, 16-18 hours), using APP Antibody. Western blot分析未处理和nocodazole处理（1ug/ml，16-18小时）的HeLa（人）、3T3L1（小鼠）和C6（大鼠）细胞提取物，使用的抗体是APP Antibody 兔多抗。 | |
Confocal microscopic images of SH-SY5Y cells showing cytoplasmic and ER stain with APP Antibody (A, red) compared to an isotype control (B). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). |
