| 货号 | 12301S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | APC2 Antibody recognizes endogenous levels of total APC2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin that has been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1/FZR1, which contain a C-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Western blot analysis of extracts from various cell lines using APC2 Antibody. Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human APC2 (hAPC2-Myc/DDK; +), using APC2 Antibody. Western blot检测mock transfected (-)或转染有表达Myc/DDK-tagged全长人APC2 (hAPC2-Myc/DDK; +)293T细胞。 | |
Immunoprecipitation of APC2 from 293T cell extracts, using Normal Rabbit IgG #2729 (lane 2) or APC2 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using APC2 Antibody. Immunoprecipitation 检测293T细胞提取物，采用Normal Rabbit IgG #2729 (lane 2) 或APC2 抗体(lane 3). Lane 1中样品浓度为10%。Western blot采用APC2 Antibody。 |
