| 货号 | 2504S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | APC Antibody detects endogenous levels of total APC protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The Adenomatous Polyposis Coli (APC) tumor suppressor gene is mutated in most familial and sporadic colorectal cancers and encodes a large cytoplasmic protein that is implicated in cell migration, cell adhesion, and proliferation (1). APC binds directly to microtubules and lack of APC leads to defective mitotic spindles and aneuploidy due to missegregation of chromosomes (2). APC is well characterized as a scaffolding protein, binds to β-catenin, and is involved in the regulation of its intracellular concentration. In the absence of a Wnt signal, GSK-3β phosphorylates all three members of the APC-β-catenin-axin complex and this phosphorylation of β-catenin creates a recognition site for ubiquitin, the signal for proteasome-mediated degradation. In the presence of a Wnt signal, dishevelled inactivates GSK-3β and β-catenin coordinates gene transcription of proteins important for the control of cell cycle progression and proliferation, such as cyclin D1 and c-Myc (3). |
| 存放说明 | -20C |
| 计算分子量 | 160 truncated APC in SW480 cells. 310 full-length APC. |
Western blot analysis (lane 1) and Immunoprecipitation (lanes 2-3) of extracts from SW480 cells. APC was immunoprecipitated with APC Antibody (lane 2) or with rabbit IgG (lane 3) to confirm specificity. Western blot was detection was performed using APC Antibody. 对SW480细胞提取物进行western blot分析（第一道）和免疫沉淀实验（第2-3道）。APC与APC抗体（第二道）或IgG（第三道）进行免疫沉淀以确保特异性。使用APC抗体进行western blot分析。 |
