| 货号 | 4059S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Akt3 Antibody detects endogenous levels of total Akt3, but does not recognize the truncated form of rat Akt3. The antibody does not cross-react with recombinant Akt1 or Akt2. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of immunoprecipitates from MDA-MB-231 cell lysates. Akt1 was immunoprecipitated with Akt1 (2H10) Monoclonal Antibody #2967, Akt 2 was immunoprecipitated with Akt2 Antibody #2962, Akt 3 was immunoprecipitated with Akt3 Antibody, and specificity was confirmed by immunprecipitation with rabbit IgG. Immunoblot was performed using Akt1 Antibody #2967 (left), Akt2 Antibody #2962 (middle) and Akt3 Antibody #4059 (right). western blot 分析MDA-MB-231 细胞裂解物的免疫沉淀。Akt1采用Akt1 (2H10) Monoclonal Antibody #2967沉淀，Akt2采用Akt2 Antibody #2962沉淀，Akt3采用 Akt3 Antibody沉淀。特异性采用兔源IgG进行免疫沉淀确定。免疫印迹使用Akt1 Antibody #2967 (左), Akt2 Antibody #2962 (中) 和Akt3 Antibody #4059 (右)。 | |
Western blot analysis (lane 1) and Immunoprecipitation (lanes 2-5) of extracts from HA-Akt3-transfected HeLa cells. HA-Akt3 was immunoprecipitated with HA-Tag (6E2) Mouse mAb #2367 (lane 2) or with Akt3 Antibody (lane 4). Immunprecipitation with mouse IgG (lane 3) and rabbit IgG (lane 5) confirm specificity. Immunoblot was performed using HA-Tag (6E2) Mouse mAb #2367. western blot 分析（泳道1） HA-Akt3-转染的 HeLa 提取物的免疫沉淀（泳道2-5）.HA-Akt3 采用HA-Tag (6E2) Mouse mAb #2367 (泳道 2) or with Akt3 Antibody (泳道 4)沉淀. 采用鼠源IgG（泳道3） 和兔源IgG（泳道5）的免疫沉淀验证特异性。免疫印迹采用HA-Tag (6E2) Mouse mAb鼠单抗 #2367进行。 | |
Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins, and extracts from MDA-MB-231 cells, using Akt3 Antibody (upper) or Akt Antibody #9272 (lower). Recombinant Akt3 is a 6His-fusion protein, MW=66 kDa. Western blot 分析重组的Akt1，Akt2， Akt3蛋白，以及MDA-MB-231细胞提取物，使用的抗体为Akt3 Antibody(上图) 或者 Akt Antibody #9272 (下图).。重组的Akt3是6His融合蛋白， MW=66 kDa。 | |
Western blot analysis of extracts from CAD and PC12 cells, using Akt3 Antibody. Western blot分析CAD和PC12细胞提取物，所用抗体为AKT3 antibody。 |
