| 货号 | 13035S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | AKR1C2 Antibody recognizes endogenous levels of total AKR1C2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Aldo-keto reductase family 1 member C2 (AKR1C2) is a member of a large superfamily of aldo-keto reductases that metabolize an array of substrates in a NADPH-dependent manner. AKR1C2 is the 3-α-hydroxysteroid dehydrogenase (3α-HSD) responsible for metabolizing and inactivating the androgen hormone 5α-dihydrotestosterone (DHT) to 3α-androstanediol (1). Research studies demonstrate an increase in AKR1C2 expression in cases of advanced prostate cancer (2). Both AKR1C2 and the related reductase, AKR1C3, are implicated in enhancing PI3K/Akt signaling in prostate cancer by reducing prostaglandin D2 to the more stable 9α, 11β-PGF2α (2). In addition, research studies demonstrate an increase in AKR1C2 expression in some ovarian endometriosis specimens, suggesting the participation of AKR1C2 in the promotion of progesterone metabolism in ovarian endometriosis (3). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from MCF7 and MCF 10A cell lines and human adipocytes using AKR1C2 Antibody. 使用AKR1C2抗体对MCF7和MCF 10A细胞系及人脂肪细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human AKR1C2 (hAKR1C2-Myc/DDK; +), using AKR1C2 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). 模拟转染(-)或转染了表达Myc/DDK标记的full-length human AKR1C2 (hAKR1C2-Myc/DDK; +)质粒的293细胞提取物，使用AKR1C2 Antibody (上), DYKDDDDK Tag (9A3) Mouse mAb 鼠单抗#8146 (中), 或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下)进行western blot分析。 |
