| 货号 | 4897S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Ajuba Antibody detects endogenous levels of total Ajuba protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from P19 cells (lane 1) and COS-7 cells transfected with Ajuba (lane 2), using Ajuba Antibody.对P19细胞（第一道）和转染了Ajuba的COS-7细胞（第二道）提取物使用Ajuba抗体进行western blot分析。 | |
Western blot analysis of extracts from MEF -/- cells rescued with a construct overexpressing Ajuba (lane 1), MEF -/- cells (lane 2), MEF +/+ cells (lane 3) and P19 cells (lane 4), using Ajuba Antibody. (Kindly provided by Dr. Gregory D. Longmore, Washington University, St. Louis, Missouri.)过表达Ajuba进行挽救实验的MEF-/-细胞（第1道），MEF -/- 细胞 (第2道), MEF +/+ 细胞cells (第 3道)和P19细胞（第4道）提取物使用Ajuba抗体进行western blot分析（由密苏里州圣路易斯华盛顿大学Gregory D. Longmore博士友情提供）。 |
