| 货号 | 9638S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | 14-3-3 tau Antibody detects endogenous levels of total 14-3-3 tau protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes which modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.使用14-3-3 τ Antibody，免疫印迹（Western Blot）分析HeLa、NIH/3T3和C6细胞系中14-3-3 τ蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.使用14-3-3 tau Antibody，免疫组化分析人肺癌组织石蜡切片，结果显示在细胞质和细胞核内定位。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).使用14-3-3 tau Antibody，免疫组化分析人类乳腺癌组织石蜡切片，其分别孵育control peptide (左图)和antigen-specific peptide (右图)。 | |
Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.使用14-3-3 tau Antibody，免疫组化分析人GIST组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.使用14-3-3 tau Antibody，免疫组化分析人乳腺癌组织石蜡切片。 |
