| 货号 | 68492S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Androgen Receptor (AR-V7 Specific) Antibody recognizes endogenous levels of total AR-V7 protein. This antibody does not cross-react with full-length AR protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3). The AR3 or AR-V7 isoform, which lacks the typical ligand binding domain, is created through the alternative splicing of cryptic exons (4-5). AR-V7 is frequently expressed in castration-resistant prostate cancer (CRPC) and while dependent on the activity of the full-length androgen receptor (AR-FL), AR-V7 can activate a completely distinct transcriptional program (6-8). While enzalutamide and abiraterone have been beneficial in treating CRPC through the ligand binding domain of AR-FL, resistance in patients has been shown to be associated with AR-V7 detection in circulating tumor cells (9-12). Studies probing into mechanisms of overcoming this resistance are currently being explored and may help in stratifying patient populations for more personalized therapies (13-15). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8. 2 . Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142. 3 . Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146. 4 . Hu, R. et al. (2009) Cancer Res 69, 16-22. 5 . Guo, Z. et al. (2009) Cancer Res 69, 2305-13. 6 . Watson, P.A. et al. (2010) Proc Natl Acad Sci U S A 107, 16759-65. 7 . Sun, S. et al. (2010) J Clin Invest 120, 2715-30. 8 . Hu, R. et al. (2012) Cancer Res 72, 3457-62. 9 . Scher, H.I. et al. (2012) N Engl J Med 367, 1187-97. 10 . de Bono, J.S. et al. (2011) N Engl J Med 364, 1995-2005. 11 . Ryan, C.J. et al. (2013) N Engl J Med 368, 138-48. 12 . Antonarakis, E.S. et al. (2014) N Engl J Med 371, 1028-38. 13 . Liu, C. et al. (2014) Clin Cancer Res 20, 3198-210. 14 . Sarwar, M. et al. (2016) Oncotarget 7, 63065-63081. 15 . Ku, S.Y. et al. (2017) Science 355, 78-83. |
Western blot analysis of extracts from various cell lines using Androgen Receptor (AR-V7 Specific) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Signal corresponding to the androgen receptor V7 isoform is detected in 22Rv1 and VCAP cells (AR-V7-positive), but is not detected in LNCaP and DU 145 cells (AR-V7-negative), confirming specificity of the antibody. |
