| 货号 | 45084S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | PRAME Antibody recognizes endogenous levels of total PRAME protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). PRAME (preferentially expressed antigen in melanoma) is a cancer/testis antigen not normally expressed in any tissues except testis, but is upregulated in tumors. PRAME is expressed in melanoma cells and is recognized by cytolytic T-cells (4). It is also upregulated in other diseases, such as synovial sarcoma (5), NSCLC (6), and breast cancer, where it is thought to contribute to tumorigenesis and metastasis (7). PRAME is also highly expressed in liquid tumors such as AML (8) and can be predictive of clinical outcome in some circumstances (9). PRAME and other cancer/testis antigens are currently being pursued as novel immunotherapy targets and diagnostic biomarkers (10). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Caballero, O.L. and Chen, Y.T. (2009) Cancer Sci 100, 2014-21. 2 . De Smet, C. et al. (1999) Mol Cell Biol 19, 7327-35. 3 . Gjerstorff, M.F. et al. (2015) Oncotarget 6, 15772-87. 4 . Ikeda, H. et al. (1997) Immunity 6, 199-208. 5 . Iura, K. et al. (2017) Hum Pathol 61, 130-139. 6 . Gunn, R.B. and Fröhlich, O. (1989) Methods Enzymol 173, 54-80. 7 . Sun, Z. et al. (2016) Gene 594, 160-164. 8 . Qin, Y.Z. et al. (2017) Oncol Lett 13, 2823-2830. 9 . Oehler, V.G. et al. (2009) Blood 114, 3299-308. 10 . Salmaninejad, A. et al. (2016) Immunol Invest 45, 619-40. |
Western blot analysis of extracts from various cell lines using PRAME Antibody. | |
Immunoprecipitation of PRAME from K-562 cell extracts using Normal Rabbit IgG #2729 (lane 2) or PRAME Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PRAME Antibody and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127. |
