| 货号 | 92921S |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | LAMC1 antibody recognizes endogenous levels of total LAMC1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of alpha (LAMA), beta (LAMB), and gamma (LAMC) chains. LAMC1 (laminin gamma-1) is a ubiquitously expressed gamma chain, and has three distinct functional domain structures. The N-terminal LN domain binds to specific alpha and beta chains to form a unique laminin heterotrimer. The LE domain mediates interactions with nidogens, while the C-terminal coil-coil fragment is necessary for agrin association. Collectively, these interactions are critical for stabilization and function of the BM (1). Genetic ablation of LAMC1 has demonstrated a critical role for this protein in embryo implantation, lung and kidney development, and neuronal Schwann cell myelination and regeneration, in addition to Trypanosome infection (2-5). Upregulation of LAMC1 has also been associated with tumor progression in multiple tumor types (6-9), possibly by creating a BM environment that is favorable for cancer cell metastasis and invasion. |
| 存放说明 | -20C |
| 计算分子量 | 220-250 |
| 参考文献 | 1 . Yurchenco, P.D. (2011) Cold Spring Harb Perspect Biol 3, a004911 2 . Ramathal, C. et al. (2011) J Biol Chem 286, 19860-71. 3 . Willem, M. et al. (2002) Development 129, 2711-22. 4 . Chen, Z.L. and Strickland, S. (2003) J Cell Biol 163, 889-99. 5 . Nde, P.N. et al. (2012) Front Immunol 3, 337. 6 . Liétard, J. et al. (1997) Am J Pathol 151, 1663-72. 7 . Nishikawa, R. et al. (2014) Int J Oncol 45, 401-10. 8 . Huang, S.X. et al. (2016) Int J Oncol 49, 217-24. 9 . Kashima, H. et al. (2015) Gynecol Oncol 139, 338-44. |
Western blot analysis of extracts from various cell lines using LAMC1 Antibody. | |
Immunoprecipitation of LAMC1 protein from U118 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is LAMC1 Antibody. Western blot analysis was performed using LAMC1 Antibody. |
