Sleeping Beauty Transposase Antibody
货号：
98923S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 98923S |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Sleeping Beauty Transposase Antibody recognizes transfected levels of total Sleeping Beauty transposase protein. |
| 使用方法 | Western Blotting (1:1000)
|
性能| 供应商 | CST |
| 灵敏度 | Recombinant |
| 背景 | Sleeping Beauty Transposase is part of a transposon system designed to allow viral free genetic insertion into vertebrate DNA. The system is composed of two components: a transposable element (transposon) that can carry DNA of interest, and a transposase that cuts and pastes the transposon into the genome. The transposase was identified from a consensus sequence of inactive Tc1/mariner-like transposase DNA sequences from salmonid fish. It was constructed by fusing and modifying two sequences from Atlantic salmon (Salmo salar) and one sequence from rainbow trout (Oncorhynchus mykiss). The transposon,T, was identified from a consensus sequence of extinct Tc-1 like transposons in salmonid fish (Tanichthys albonubes) (1). Further modifications of the system have been made since its initial construction: sequence changes to the transposase to fit better to an improved consensus sequence has increased its efficiency by 100 fold (2). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Ivics, Z. et al. (1997) Cell 91, 501-10.
2 . Mátés, L. et al. (2009) Nat Genet 41, 753-61.
|
参考图片Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK tagged Sleeping Beauty Transposase (+), using Sleeping Beauty Transposase Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
