| 货号 | 17402S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Nectin-4/PVRL4 Antibody recognizes endogenous levels of total Nectin-4/PVRL4 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with PVR, PVRL1, PVRL2, and PVRL3 proteins. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nectin-4/Poliovirus Receptor-Like 4 (PVRL4) is a type-I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and promotes cell-cell adhesion by serving as a major component of adherens junctions (1-3). The extracellular domain of Nectin-4, which contains an Ig variable-like domain (V) and two Ig constant-like domains (C), mediates binding to the measles virus (4) and to neighboring cells through trans heterophilic interactions with Nectin-1 (5,6). Unlike other nectin family members, which are widely expressed in adult tissues, Nectin-4 expression in humans is largely restricted to the placenta (7). Research studies have demostrated that Nectin-4 is overexpressed in a variety of human solid tumors of the pancreas (8), breast (9,10), lung (11), and ovary (12). Due to its restricted expression pattern in normal human tissues, Nectin-4 may serve as a novel diagnostic and therapeutic target for a variety of human tumors. |
| 存放说明 | -20C |
| 计算分子量 | 62, 75 |
| 参考文献 | 1 . Takai, Y. et al. (2008) Annu Rev Cell Dev Biol 24, 309-42. 2 . Takai, Y. and Nakanishi, H. (2003) J Cell Sci 116, 17-27. 3 . Harrison, O.J. et al. (2012) Nat Struct Mol Biol 19, 906-15. 4 . Mühlebach, M.D. et al. (2011) Nature 480, 530-3. 5 . Mateo, M. et al. (2014) J Virol 88, 14161-71. 6 . Fabre, S. et al. (2002) J Biol Chem 277, 27006-13. 7 . Reymond, N. et al. (2001) J Biol Chem 276, 43205-15. 8 . Nishiwada, S. et al. (2015) J Exp Clin Cancer Res 34, 30. 9 . Lattanzio, R. et al. (2014) Oncogenesis 3, e118. 10 . Fabre-Lafay, S. et al. (2007) BMC Cancer 7, 73. 11 . Takano, A. et al. (2009) Cancer Res 69, 6694-703. 12 . Nabih, E.S. et al. (2014) Biomarkers 19, 498-504. |
Western blot analysis of extracts from HT-1376 cells, untreated (-) or treated with PNGase F (+), using Nectin-4/PVRL4 Antibody. | |
Western blot analysis of extracts from various cell lines using Nectin-4/PVRL4 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, Nectin-4/PVRL4 expression is not detected in either MDA-MB-231 or A-549 cells. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Nectin-4/PVRL4 protein (hNectin-4/PVRL4-Myc/DDK; +), using Nectin-4/PVRL4 Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
