| 货号 | 17327S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | CD151 Antibody recognizes endogenous levels of total CD151 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD151 (PETA-3, SFA-1) is a member of the evolutionarily conserved tetraspanin family of multipass glycoproteins (TM4SF), highlighted by four transmembrane domains, two extracellular loops, and N/C-termini that reside within the cytoplasm. Identified as the first member of the tetraspanin family to be implicated in tumorigenesis, research studies have demonstrated that CD151 participates in tumor neovascularization (1), tumor cell cell invasion (2), and cell adhesion (3). Furthermore, a positive correlation exists between CD151 expression levels and poor prognosis for tumors of the lung (4), kidney (5), and prostate (6). CD151 is localized predominantly to the plasma membrane and research studies have demonstrated that CD151 exerts its pro-tumorigenic effects, in part, through the modulation of laminin-binding integrins (7-9) and oncogenic receptor tyrosine kinases, such as c-Met (10,11) and EGFR (12). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
| 参考文献 | 1 . Takeda, Y. et al. (2007) Blood 109, 1524-32. 2 . Yauch, R.L. et al. (1998) Mol Biol Cell 9, 2751-65. 3 . Zhang, F. et al. (2011) Blood 118, 4274-84. 4 . Tokuhara, T. et al. (2001) Clin Cancer Res 7, 4109-14. 5 . Yoo, S.H. et al. (2011) Histopathology 58, 191-7. 6 . Ang, J. et al. (2004) Cancer Epidemiol Biomarkers Prev 13, 1717-21. 7 . Sincock, P.M. et al. (1999) J Cell Sci 112 ( Pt 6), 833-44. 8 . Winterwood, N.E. et al. (2006) Mol Biol Cell 17, 2707-21. 9 . Liu, L. et al. (2007) J Biol Chem 282, 31631-42. 10 . Klosek, S.K. et al. (2009) Biochem Biophys Res Commun 379, 1097-100. 11 . Franco, M. et al. (2010) J Biol Chem 285, 38756-64. 12 . Yang, X.H. et al. (2008) Cancer Res 68, 3204-13. |
Western blot analysis of extracts from various cell lines using CD151 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD151 expression is not detected in Daudi cells. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD151 protein (hCD151-Myc/DDK; +), using CD151 Antibody (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
