| 货号 | 79299S |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | Angiotensinogen Antibody recognizes endogenous levels of total angiotensinogen protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Angiotensinogen (AGT) is the primary precursor of angiotensins, peptide hormones that play a central role in the renin-angiotensin system (RAS) (1-3). AGT is a secreted protein synthesized primarily by the liver and secreted into circulation. Upon binding to renin, the amino terminal fragment of AGT is cleaved and released as a decapeptide hormone termed angiotensin 1 (Ang I). Ang I is subsequently processed by angiotensin converting enzyme (ACE) to generate angiotensin II (Ang II), which acts on AT1 and AT2 receptors in the central nervous system to increase production of anti-diuretic hormone (ADH), while promoting vasoconstriction in the peripheral circulation (4). Aberrant upregulation of Ang II has been associated with numerous clinical conditions, including hypertension, atherosclerosis, myocardial hypertrophy, and obesity (5-7). Alternative cleavage products of Ang I (e.g., Ang 1-7) can also be generated by ACE2 cleavage, some of which display biological functions that are distinct from Ang II (8). Treatments that target the RAS (e.g., ACE inhibitors) are consequently of significant importance in the treatment of hypertensive and hypertensive-related disorders(5-8). |
| 存放说明 | -20C |
| 计算分子量 | 55, 70 |
| 参考文献 | 1 . Lu, H. et al. (2016) Hypertens Res 39, 492-500. 2 . Sparks, M.A. et al. (2014) Compr Physiol 4, 1201-28. 3 . Kumar, R. et al. (2012) Clin Sci (Lond) 123, 273-84. 4 . de Kloet, A.D. et al. (2015) Am J Physiol Regul Integr Comp Physiol 309, R444-58. 5 . Vajapey, R. et al. (2014) Front Physiol 5, 439. 6 . Wu, C. et al. (2011) N Am J Med Sci (Boston) 4, 183-190. 7 . Putnam, K. et al. (2012) Am J Physiol Heart Circ Physiol 302, H1219-30. 8 . Padda, R.S. et al. (2015) J Diabetes Metab6. |
Western blot analysis of extracts from Hep G2, Huh7, and Hep3B2.1-7 cell lines using Angiotensinogen Antibody. | |
Immunoprecipitation of angiotensinogen protein from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Angiotensinogen Antibody. Western blot analysis was performed using Angiotensinogen Antibody and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP conjugated) #5127. |
